Chickpea root DNA tool to identify chickpea root distribution

Abstract

Key findings •A new, advantageous DNA-based chickpea root measurement method was developed as an alternative to other root quantification methods that allows large numbers of soil cores to be analysed over a short time period. •Field results showed the DNA method could be used to determine chickpea root depth distribution. •The results also showed that differences in root dry matter distribution and soil water extraction exist among chickpea varieties and breeding lines. •Comparing genotypes using the DNA method is complex due to differences in DNA copy number between genotypes; currently the method is most readily used to determine factors affecting root distribution in a single chickpea genotype.