In-silico oligonucleotide primers developed for improving DNA barcoding of the stored grains pest khapra beetle Trogoderma granarium (Coleoptera: Dermestidae)

Abstract

Key findings •The significant pest khapra beetle can be identified by their DNA barcodes for improved biosecurity outcomes. •Five novel oligonucleotide primers were designed to improve specificity and amplification of DNA barcodes from khapra beetle and related taxa. •The primers will enhance using DNA barcoding for verifying khapra beetle when detected by other diagnostic protocols.